Consistent high-quality DNA purification from bacteria, yeast, mushroom, fungi and plant (leaf, rice, potato)
Performance specifications
| Sample | Sample amount | DNA yield (µg) | 260/280 ratio |
|---|---|---|---|
| Bacteria | 107-109 cells | 3-20 | 1.7-2.0 |
| Mycobacteria | 107-108 cells | 3-18 | 1.7-1.9 |
| Yeast | 107-109 cells | 4-15 | 1.7-2.0 |
| Fungi | 2-10 mg | 1-5 | 1.6-1.8 |
| Mushroom | 5-20 mg | 3-30 | 1.8-2.0 |
| Plant (Leaf) | 10-20 mg | 3-42 | 1.7-1.9 |
| Plant (Rice) | 2-10 grains | 3-25 | 1.7-2.0 |
| Plant (Potato) | 10-20 mg | 10-20 | 1.8-2.0 |
Faster, easier, low-cost DNA extraction
Protocols and examples of DNA isolated from various samples
Sample preparation for DNA extraction from bacteria
Use bacteria cells from overnight culture after centrifugation and discarding the media, or colonies from a culture plate. Use 107-109 cells or 5-20 mg of culture.
To the right is a graph showing DNA extraction from 4 different samples of bacteria using Swagene Universal DNA kit and measured by a nanospectrophotometer.
Sample preparation for DNA extraction from yeast
Use yeast cells from overnight culture after centrifugation and discarding the media, or colonies from a culture plate. Use 107-108 cells or 5-20 mg of culture for DNA isolation.
To the right is a graph showing DNA extraction from 4 different samples of yeast using Swagene Universal DNA kit and measured by a nanospectrophotometer.
Sample preparation for DNA extraction from fungi
Use fungal cells from culture after centrifugation and discarding the media, or colonies from a culture plate. Use 107-108 cells or 5-10 mg of culture.
Sample preparation for DNA extraction from mushroom
Use 5-20 mg of mushroom for DNA isolation .
To the right is a graph showing DNA extraction from 4 different samples of mushroom using Swagene Universal DNA kit and measured by a nanospectrophotometer.
Sample preparation for extraction of DNA from plant (leaf, rice, potato)
Grind 5-20 mg leaves or rice or potato in a mortar and pestle in the presence of liquid nitrogen or after cooling it on dry ice. Use 5-20 mg of ground leaves for DNA isolation.
Below are the graphs showing DNA extraction from 4 different samples of leaf (left), 3 different samples of rice (middle) and 4 different samples of potato (right) using Swagene Universal DNA kit and measured by a nanospectrophotometer.
 |  |  |
|---|
Downstream applications
High yield and quality of DNA or RNA enable use in several specialized downstream applications
Kit Contents (50 isolations)
- Lysis Buffer (LD-U)
- Wash Buffer, concentrate* (WD)
- Elution Buffer (ED)
- DNA spin columns
- Collection Tubes
- Abridged user manual
* Before use, add 100% ethanol to the concentrated wash buffer to make working WD as specified on the bottle